Elucidation of cell subsets in pathogen-induced VAT-inflammation and their role in onset of IR and GI
To accomplish research objective 2, we will make use of genetically modified mice.
These include mice that lack activating immune-cell receptors (NKG2D, NKp46, Ly49, TNF-R), pro-inflammatory proteins (IFNα –β or –γ, perforin, granzyme A/B), T cells, B cells or both (JHT-/-, TCRα-/-, SCID).
Our genetic models will be combined with ‘standard’ immunological models (adoptive transfers, bone marrow chimeras, antibody depletion, etc.).